TaqMan® MGB (minor groove binder) probes incorporate a 5′ reporter dye and a 3′ nonfluorescent quencher (NFQ). The NFQ offers the advantage of lower background signal, which results in better precision of quantification. Custom TaqMan MGB probes are HPLC-purified and available with FAM™, VIC™, TET™, and NED™ reporter dyes.
Minor Groove Binder modification enhances detection specificity
TaqMan MGB probes include a minor groove binder (MGB) moiety attached to the NFQ at the 3’ end that increases the melting temperature (Tm) of the probe and stabilizes probe–target hybrids. This means that TaqMan MGB probes can be significantly shorter than traditional probes, providing better sequence discrimination and flexibility to accommodate more targets.
Nonfluorescent quencher (NFQ) maximizes sensitivity
TaqMan MGB probes incorporate an NFQ to absorb (quench) signal from the fluorescent dye label at the other end of the probe. The properties of the NFQ combined with the short length of the MGB probe result in lower background signal than with non-MGB NFQ probes. Lower background means increased sensitivity and precision in your data.
Figure 1: A fluorescent reporter (R) dye and a quencher (NFQ-MGB) are attached to the 5′ and 3′ ends of TaqMan® probe, respectively.
Click here for more detailed information about Taqman MGB Probes.
Custom Taqman MGB Probes can be applied in variable qPCR experiments types, such as: gene expression, miRNA expression, genotyping (SNP analysis) and copy number variation (CNV).
Feel free to contact us via RT-PCR@rhenium.co.il, and we would be happy to help you with Taqman MGB Probes designing.
Custom Taqman MGB Probe (4316034)
set Primers (4304970)