We are celebrating three years for our real time PCR newsletter and as a loyal reader, we would like to thank you and provide you reagents (TaqMan or SYBR mixes) for real time PCR for one year for free*!

Speaking of reagents – how would you know Which real-time PCR chemistry is right for you?

 SYBR or TaqMan?

Choosing the right chemistry and master mix is an important step when performing qPCR experiment.   A master mix is a mixture of polymerase, salts, magnesium, dNTPs, and optimized reaction buffer. Your template and primers are the only additions necessary to perform qPCR. Different formulations are good for different situations or applications such as Genotyping, gene expression, MicroRNA quantification, and virus detection.

We offer two main types of detection technologies when performing real-time PCR:
SYBR® Green and TaqMan® chemistry.

SYBR® Green
based on two hybridization events, the forward and reverse primers.

Advantages of SYBR dye

  • It can be used to monitor the amplification of any double-stranded DNA sequence.
  • No probe is required, which can reduce assay setup and running costs, assuming that your PCR primers are well designed, and your reaction is well characterized.

Disadvantage of SYBR dye

The primary disadvantage is that it may generate false positive signals, i.e., because the SYBR dye binds to any double-stranded DNA, it can also bind to nonspecific double-stranded DNA sequences. Therefore, it is extremely important to have well-designed primers that do not amplify non-target sequences, and that melt curve analysis be performed.

TaqMan® chemistry

TaqMan master mix which bases its detection on 5’ nuclease activity. requires three hybridization events: two PCR primers and one probe, with hydrolysis of the probe during extension of the primers.

TaqMan® chemistry offers the convenience of millions of pre-designed assays and greater levels of specificity. 

Advantages of TaqMan chemistry

  • Specific hybridization between probe and target is required to generate fluorescent signal
  • Probes can be labeled with different, distinguishable reporter dyes, which allows amplification and detection of two distinct sequences in one reaction tube
  • Post-PCR processing is eliminated, which reduces assay labor and material costs

Disadvantage of TaqMan chemistry

The primary disadvantage is that the synthesis of different probes is required for different sequences. If you need high specificity and a predesigned assay for gene expression experiment you better choose Taqman® chemistry.
In order to choose the right master mix, insert the online selection tool.

Special Offer*

Buy a real time PCR instrument from the participating models** and get reagents for free for one year!!!

**The participating models are:
 QuantStudio 3 and QuantStudio 5 Real-Time PCR Systems.
The number of supplying reagents (TaqMan or SYBR mixes) related to average consumption at Israeli market.