● Absolute Q is intended to be more sensitive and precise than conventional qPCR.
● The dPCR workflow is identical to the qPCR workflow you are familiar with to improve ease of use, minimize hands on steps, and maximize consistency.
It’s all about Compartmentalization:
dPCR is a method of quantifying without standard curves, by dividing the bulk reaction into thousands of smaller, independent reactions.
The Endpoint is an Absolute quantification which enables greater precision, reactions are categorized as positive or negative at endpoint
QuantStudio Absolute Q Digital PCR System
Product Brochure: QuantStudio Absolute Q Digital PCR System
Applications of dPCR:
Viral vector and plasmid development for cell and gene therapy
dPCR offers significant advantages for quantification of target DNA molecules in a range of analytical assays necessary for viral vector production and characterization.
Analyze more samples and improve sensitivity for rare targets by pooling digital PCR data
This method could be used for a multitude of rare-target detection applications in the precision medicine space, such as monitoring treatment response, screening for minimal residual disease, and rapid identification of mutation-linked drug resistance.
dPCR as a quantification tool for NGS libraries
Utilizing dPCR as a quantification tool for NGS libraries before sequencing helps optimize sequencing run performance, data generation, and data quality. dPCR, which gives absolute rather than relative quantification, is more accurate and precise at low concentrations than qPCR.